The Global Polio Surveillance Action Plan 2025–2026 defines the surveillance activities required to achieve the goals of the Global Polio Eradication Initiative (GPEI) for the interruption of wild poliovirus type 1 (WPV1) transmission and outbreaks of circulating vaccine-derived poliovirus type 2 (cVDPV2). The Global Polio Surveillance Action Plan (GPSAP) brings together the five surveillance work streams that collectively function to help guide the polio eradication effort, namely: 1) syndromic surveillance for cases of acute flaccid paralysis (AFP) in children aged under 15 years, referred to as AFP surveillance; 2) surveillance for poliovirus in sewage and wastewater, referred to as environmental surveillance; 3) surveillance for immunodeficiency-associated vaccine-derived poliovirus in patients with primary immunodeficiency disorders, referred to as iVDPV surveillance; 4) laboratory testing to provide confirmation and genetic sequencing of polioviruses, provided by the Global Polio Laboratory Network; and 5) data and information management that support reporting detections and monitoring poliovirus surveillance via a centralized repository, referred to as the Polio Information System or POLIS. Lessons learned from the earlier Global Polio Surveillance Action Plan 2022–2024 inform changes in the new action plan. This GPSAP also continues to align with and support polio surveillance objectives and activities detailed in the GPEI Polio Eradication Strategy.