Protein Liquid Chromatography
M. Kastner
Π΄Π΅ΠΊ. 1999β―Π³. Β· Journal of Chromatography Library ΠΠ½ΠΈΠ³Π° 61 Β· Elsevier
Π-ΠΊΠ½ΠΈΠ³Π°
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reportΠΡΠ΅Π½ΠΈΡΠ΅ ΠΈ ΡΠ΅ΡΠ΅Π½Π·ΠΈΠΈΡΠ΅ Π½Π΅ ΡΠ΅ ΠΏΠΎΡΠ²ΡΠ΄Π΅Π½ΠΈ Β ΠΠΎΠ·Π½Π°ΡΡΠ΅ ΠΏΠΎΠ²Π΅ΡΠ΅
ΠΠ° Π΅-ΠΊΠ½ΠΈΠ³Π°Π²Π°
Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.
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