Neutron Protein Crystallography: Hydrogen, Protons, and Hydration in Bio-macromolecules
Nobuo Niimura · Alberto Podjarny
feb. 2011 · International Union of Crystallography Monographs on Crystallography Cartea 25 · Oxford University Press
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This is one of the first books dedicated to the emerging field of neutron protein crystallography (NPC). The text covers all of the practical aspects of NPC, from the basic background of neutron scattering and diffraction, to the technical details of neutron facilities, growth of high-quality crystals, and data analysis. The final chapter is devoted to providing many examples of using NPC to investigate a wide range of different proteins. It demonstrates how NPC can explore hydrogen bonds, protonation and deprotonation of amino acid residues, hydration structures, and hydrogen-to-deuterium exchange ratios. To avoid redundancy with other textbooks on X-ray protein crystallography (XPC), this book assumes a familiarity with the basics of XPC and strives to highlight and explain the differences between XPC and NPC. It is therefore especially useful for X-ray protein crystallographers who are eager to have a sound, scientific basis for judging if NPC is the right technique for furthering their experimental programs.
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Nobuo Niimura took his PhD from the University of Tokyo in 1970. He has since worked in the Physics Division, Tohoku University, and the Japan Atomic Energy Research Institute. He is currenlty Professor at the Frontier Research Center for Applied Atomic Sciences, Ibaraki University. He has received a number of awards for hiw work: Tsukuba Science Award (1996), Nikkei BP Award (1998), Award of Radiation of Applied Physics Society of Japan (1998), Award of Ministry of Science & Technology Agency (1999), Award of Neutron Science Society of Japan (2003) Alberto Podjarny began his research career at La Plata University in Argentina. he has spent time as a Visiting Associate Professor, and Research Associate at the Departments of Biophysics and Theoretical Biology, and Biochemistry and Molecular Biology, University of Chicago. He is currently Director of Research for CNRS, France.
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