Isolation and Purification of Proteins
Rajni Hatti-Kaul · Bo Mattiasson
Feb 2003 · CRC Press
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This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
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Rajni Hatti-Kaul is Senior Lecturer, Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Sweden. The author or coauthor of 60 scientific papers, several book chapters, and two books, she received the M.Sc. (1979) and the Ph.D. (1984) degrees from the University of Bombay, India. Bo Mattiasson is Professor, Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Sweden. The author or coauthor of more than 550 professional publications and five books, he received the M.Sc. (1971) and Ph.D. (1974) degrees from Lund University, Sweden.
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