Heterologous Expression of Membrane Proteins: Methods and Protocols, Edition 3
Isabelle Mus-Veteau
ΡΡΠ½. 2022β―Π³. Β· Methods in molecular biology ΠΠ½ΠΈΠ³Π° 1 Β· Springer Nature
Π-ΠΊΠ½ΠΈΠ³Π°
467
Π‘ΡΡΠ°Π½ΠΈΡΠΈ
reportΠΡΠ΅Π½ΠΈΡΠ΅ ΠΈ ΡΠ΅ΡΠ΅Π½Π·ΠΈΠΈΡΠ΅ Π½Π΅ ΡΠ΅ ΠΏΠΎΡΠ²ΡΠ΄Π΅Π½ΠΈ Β ΠΠΎΠ·Π½Π°ΡΡΠ΅ ΠΏΠΎΠ²Π΅ΡΠ΅
ΠΠ° Π΅-ΠΊΠ½ΠΈΠ³Π°Π²Π°
This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.
Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.
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