Expression Systems: Methods Express
Michael Dyson · Yves Durocher
កញ្ញា 2007 · Scion Publishing Ltd
សៀវភៅអេឡិចត្រូនិច
288
ទំព័រ
family_home
មានសិទ្ធិ
info
reportការវាយតម្លៃ និងមតិវាយតម្លៃមិនត្រូវបានផ្ទៀងផ្ទាត់ទេ ស្វែងយល់បន្ថែម
អំពីសៀវភៅអេឡិចត្រូនិកនេះ
Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when.
Expression Systems: Methods Express uniquely fills this need. It covers expression across a broad range of systems, including the following. *Baculovirus expression vectors
*CHO cells
* E. coli
*HEK293-EBNA1 cells
* Lactococcus lactis and other gram positive bacteria
* S. cerevisiae
*transfected insect cells
* Pichia pastoris
*mammalian cells using BacMam viruses
*lentiviral vectors
*wheat germ cell-free system
The book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression.
Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench.
In summary, Expression Systems: Methods Express is a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.
Expression Systems: Methods Express uniquely fills this need. It covers expression across a broad range of systems, including the following. *Baculovirus expression vectors
*CHO cells
* E. coli
*HEK293-EBNA1 cells
* Lactococcus lactis and other gram positive bacteria
* S. cerevisiae
*transfected insect cells
* Pichia pastoris
*mammalian cells using BacMam viruses
*lentiviral vectors
*wheat germ cell-free system
The book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression.
Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench.
In summary, Expression Systems: Methods Express is a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.
វាយតម្លៃសៀវភៅអេឡិចត្រូនិកនេះ
ប្រាប់យើងអំពីការយល់ឃើញរបស់អ្នក។
អានព័ត៌មាន
ទូរសព្ទឆ្លាតវៃ និងថេប្លេត
ដំឡើងកម្មវិធី Google Play Books សម្រាប់ Android និង iPad/iPhone ។ វាធ្វើសមកាលកម្មដោយស្វ័យប្រវត្តិជាមួយគណនីរបស់អ្នក និងអនុញ្ញាតឱ្យអ្នកអានពេលមានអ៊ីនធឺណិត ឬគ្មានអ៊ីនធឺណិតនៅគ្រប់ទីកន្លែង។
កុំព្យូទ័រយួរដៃ និងកុំព្យូទ័រ
អ្នកអាចស្ដាប់សៀវភៅជាសំឡេងដែលបានទិញនៅក្នុង Google Play ដោយប្រើកម្មវិធីរុករកតាមអ៊ីនធឺណិតក្នុងកុំព្យូទ័ររបស់អ្នក។
eReaders និងឧបករណ៍ផ្សេងទៀត
ដើម្បីអាននៅលើឧបករណ៍ e-ink ដូចជាឧបករណ៍អានសៀវភៅអេឡិចត្រូនិក Kobo អ្នកនឹងត្រូវទាញយកឯកសារ ហើយផ្ទេរវាទៅឧបករណ៍របស់អ្នក។ សូមអនុវត្តតាមការណែនាំលម្អិតរបស់មជ្ឈមណ្ឌលជំនួយ ដើម្បីផ្ទេរឯកសារទៅឧបករណ៍អានសៀវភៅអេឡិចត្រូនិកដែលស្គាល់។
