Differential-Display Reverse Transcription-PCR (DDRT-PCR)
Sergio Colonna-Romano · Antonella Leone · Bruno Maresca
dic 2012 · Springer Science & Business Media
5,0star
1 recensionereport
Ebook
124
pagine
reportValutazioni e recensioni non sono verificate Scopri di più
Informazioni su questo ebook
Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.
Valutazioni e recensioni
5,0
1 recensione
Valuta questo ebook
Dicci cosa ne pensi.
Informazioni sulla lettura
Smartphone e tablet
Installa l'app Google Play Libri per Android e iPad/iPhone. L'app verrà sincronizzata automaticamente con il tuo account e potrai leggere libri online oppure offline ovunque tu sia.
Laptop e computer
Puoi ascoltare gli audiolibri acquistati su Google Play usando il browser web del tuo computer.
eReader e altri dispositivi
Per leggere su dispositivi e-ink come Kobo e eReader, dovrai scaricare un file e trasferirlo sul dispositivo. Segui le istruzioni dettagliate del Centro assistenza per trasferire i file sugli eReader supportati.
