Adhesion Protein Protocols: Edition 2

· Methods in molecular biology Libro 370 · Springer Science & Business Media
Libro electrónico
234
Páxinas
As valoracións e as recensións non están verificadas  Máis información

Acerca deste libro electrónico

The second edition of Adhesion Protein Protocols combines traditional te- niques with cutting-edge and novel techniques that can be easily adapted to different molecules and cell types. These protocols are suited for both novice and expert scientists and will hopefully be used to gain further insight into the complex and incompletely understood processes that are involved in cellular adhesion. The book begins with two chapters covering novel techniques for studying cell-cell adhesion, which traditionally has been less straightforward to examine. The first chapter by Nelson et al. describes their novel technique for studying cell-cell adhesion using bowtie-shaped microwells. This technique not only allows one to control the degree to which the cells spread, but also allows manipulation of cell-substratum interactions. In the second chapter, Vogelmann and Nelson describe the use of differential centrifugation to isolate cell-cell adhesion complexes as a useful starting point to further examine adhesion protein interactions. Chapter 3 by Nuzzi and co-workers describes the analysis of neutrophil chemotaxis, and includes methods for retroviral infection of these difficult-- transfect cell types and time-lapse video microscopy. In Chapter 4, McGettrick and colleagues describe several in vitro assays used to study leukocyte mig- tion through monolayers of cultured endothelial cells. Cell motility requires the formation of pseudopodia, and in Chapter 5, Wang and Klemke describe a novel technique to purify pseudopodia from migratory cells.

Valora este libro electrónico

Dános a túa opinión.

Información de lectura

Smartphones e tabletas
Instala a aplicación Google Play Libros para Android e iPad/iPhone. Sincronízase automaticamente coa túa conta e permíteche ler contido en liña ou sen conexión desde calquera lugar.
Portátiles e ordenadores de escritorio
Podes escoitar os audiolibros comprados en Google Play a través do navegador web do ordenador.
Lectores de libros electrónicos e outros dispositivos
Para ler contido en dispositivos de tinta electrónica, como os lectores de libros electrónicos Kobo, é necesario descargar un ficheiro e transferilo ao dispositivo. Sigue as instrucións detalladas do Centro de Axuda para transferir ficheiros a lectores electrónicos admitidos.