The 16 papers presented in this book were carefully reviewed and selected from 31 submissions. The papers cover various topics such as computer-aided detection and diagnosis of disease; image analysis of novel ophthalmic imaging modalities; multimodal ophthalmic image analysis; ophthalmic image atlases; ophthalmic image analysis in animals; registration of ophthalmic images, including multimodal, segmentation of structures (e.g., vasculature, lesions, landmarks), combined analysis of images of the eye and other organs; validation; and/or crowd sourcing.